Procedures for Reticulocyte and Platelet Count

Introduction

Reticulocytes and platelets play a crucial role in hematology, helping diagnose various blood disorders. Accurate counting of these cells is essential in assessing bone marrow function and overall blood health.

Staining Techniques for Reticulocyte Count

Supravital Stain Method

• Uses stains like New Methylene Blue or Brilliant Cresyl Blue
• Reticulocytes appear as cells containing blue-stained precipitates of RNA
• Microscopic examination helps differentiate mature RBCs from reticulocytes

Other Methods

• Fluorescent staining techniques improve accuracy
• Flow cytometry is increasingly used for precise reticulocyte quantification
• Automation enhances standardization in large-scale testing

Platelet Counting Procedures

Manual Platelet Count

Employs hemocytometer to estimate platelet numbers under a microscope.

Automated Platelet Count

Uses electronic cell counters for a more accurate and rapid assessment.

Clinical Significance

Abnormal reticulocyte and platelet counts indicate various hematological disorders, such as anemia, thrombocytopenia, or bone marrow dysfunction. Proper staining and counting techniques are essential for effective diagnosis.