Perform and Identify ABO and Rh Blood Grouping

Introduction

Blood grouping is a crucial aspect of medical science, aiding in transfusions, organ transplants, and forensic investigations. The ABO and Rh blood grouping systems help classify blood based on antigen presence.

Methodology of Rh Blood Grouping (D Antigen Detection)

Materials Required

• Antisera (Anti-D reagent)
• Glass slides/test tubes
• Microscope
• Normal saline

Procedure

1. Place a drop of Anti-D reagent on the slide.
2. Add a drop of blood sample.
3. Mix gently and observe for agglutination.

Interpretation

• Positive Agglutination: Rh-positive (D antigen present).
• No Agglutination: Rh-negative (D antigen absent).

Clinical Significance

Knowing Rh status is critical for transfusions, maternal-fetal medicine, and various medical conditions.