Procedures for Reticulocyte and Platelet Count

Methods for Platelet Count – Manual and Automated Techniques

The platelet count is a crucial diagnostic test used in assessing hematological disorders. It can be performed using both manual and automated techniques, each offering distinct advantages.

Manual Platelet Counting

Manual platelet counting is performed using a hemocytometer under a microscope. This method involves:

• Preparing a dilution of whole blood using an appropriate anticoagulant.
• Charging the hemocytometer chamber with the diluted sample.
• Counting platelets in designated grid areas.

While accurate in low platelet count cases, this method requires significant expertise and time.

Automated Platelet Counting

Modern hematology analyzers provide automated platelet counts with high precision. These analyzers use:

• Impedance technology to detect and count platelets.
• Optical fluorescence to differentiate platelets from other blood components.
• Algorithmic corrections for variations in platelet size and distribution.

Automated methods significantly reduce human error and provide rapid results, making them preferred in clinical settings.

Clinical Significance

Platelet count abnormalities can indicate various conditions:

• Thrombocytopenia: Low platelet count seen in bone marrow disorders, infections, and autoimmune diseases.
• Thrombocytosis: Elevated platelet levels observed in inflammatory disorders and malignancies.

Accurate platelet count methodologies aid in timely diagnosis and treatment planning.