Cerebrospinal Fluid (CSF) Analysis

Collection Methods: Lumbar Puncture Procedure and Precautions

Cerebrospinal fluid (CSF) analysis is a critical diagnostic procedure in pathology. It helps identify neurological disorders, infections, and other medical conditions. The lumbar puncture, commonly known as a spinal tap, is the primary method for collecting CSF.

Procedure

• Patient Positioning: The patient is positioned in a lateral decubitus or sitting posture for optimal access.
• Site Preparation: Antiseptic cleaning and sterile draping minimize infection risks.
• Needle Insertion: A specialized needle is inserted between the L3-L4 or L4-L5 vertebrae.
• Fluid Collection: CSF is collected in sterile tubes for analysis.
• Post-Procedure Care: The patient is monitored for headaches, infection, or other complications.

Precautions

• Assess for contraindications such as intracranial hypertension.
• Use sterile technique to prevent infections.
• Ensure proper hydration post-procedure.
• Monitor for adverse effects like post-lumbar puncture headache.